The C.SIGHT 2.0 is the latest iteration of our single-cell dispensing system designed to optimize cell line development (CLD) applications. With faster single-cell isolation processing times and our new EASY.ON cartridges for contamination-free dispensing, the C.SIGHT 2.0 provid...

The C.SIGHT 2.0 is the latest iteration of our single-cell dispensing system designed to optimize cell line development (CLD) applications. With faster single-cell isolation processing times and our new EASY.ON cartridges for contamination-free dispensing, the C.SIGHT 2.0 provides an easy entry point into the world of single-cell handling. The C.SIGHT 2.0’s compact design makes it possible to operate the system in biosafety cabinets, enabling single-cell isolation in sterile conditions.

Key Features & Benefits

Fast and efficient single-cell dispensing

Single cells are gently dispensed into a 96-well plate in under 2 minutes or into a 384-well plate in under 8 minutes.

No risk of contamination

Eliminate the risk of cross contamination during dispensing with single-use EASY.ON cartridges.

Assurance of clonality

Capture nozzle images for every dispensing run to assure that only single cells were dispensed in the plate.

Cell Focusing technology

Align cells at the center of the dispensing cartridge for even better handling and processing of delicate samples

Easy setup

Begin single-cell isolation in minutes thanks to the C.SIGHT 2.0’s straightforward software.

Compact design

Place the C.SIGHT 2.0 in laminar flow hoods to isolate cells in a sterile environment.

Product Details

Fast and precise dispensing

The C.SIGHT 2.0 isolates single cells in either 96- or 384-well plates quickly, dispensing an entire 384-well plate in under 8 minutes. The system’s versatility enables a multitude of different cells to be isolated.

Small footprint

Thanks to the C.SIGHT 2.0’s compact size, the device can be place inside most biosafety cabinets and connected to external control terminals, making the system’s cutting-edge technology accessible to labs of any size.

Minimizing cell loss

Add an additional layer of optimization with our patented Cell Focusing technology, which gently aligns cells at the center of the dispensing cartridge for superior cell detection. It offers significant improvements when working with rare cell types as this alignment ensures that cells are perfectly captured and measured, reducing cell loss and even increasing processing speeds.

Powerful software

Following CYTENA’s customer-oriented philosophy, the software has been designed for easy use and quick setup of individual experiments. All isolation events are recorded and imaged in full resolution, and all images are saved for future analysis and assurance of clonality.

Automation ready

The C.SIGHT 2.0 can be easily integrated into automated workflows, making it the fastest single-cell dispenser fully compatible with robotic automation.

Product Applications

Cell line development

iPSC isolation for clonal culture

Cell therapy

Gene therapy

Featured Workflow

The C.SIGHT 2.0 gently dispenses single cells înto 96- or 384-well plates. Clones of interest are then expanded and the highest producng clones are selected for downstream cell-based assays or antibody production.

Technical Specifications

Samples          

Eukaryotic cells

Cell size        

5 – 40 µm

Sorting           

Cell morphology

Droplet volume

~200 pL

Substrate

96- and 384-well plates

Nozzle imaging

Camera CMOS

Magnification 10x

Processing times

Single-cell dispensing 96-well plate: ~ 2 min

Single-cell dispensing 384-well plate: ~ 8 min

Cartridge

EASY.ON 40 µm disposable cartridge to avoid cross-contamination

Automation

Lid opening and closing

Compatible with standard automation arm and gripper

Compatible with third-party automation clients

Embedded computer

Windows

Compatibility

Standard biosafety cabinets class 2

Footprint

635 x 400 x 286 mm

Weight

40 kg

Certification

CE, CB, UL (TÜV), RoHS