Laboratory Centrifuge Operating and Maintenance Guide
Laboratory centrifuges separate components of a sample based on density through high-speed rotation. Safe operation is critical as centrifuges present significant mechanical hazards.
I. Operating Procedures
- Rotor Selection: Select the appropriate rotor for the application. Verify the rotor is compatible with the centrifuge model and the maximum speed rating is not exceeded.
- Sample Preparation: Use centrifuge tubes rated for the intended speed and temperature. Do not exceed the maximum fill volume. Ensure tube caps are properly sealed.
- Balancing: Balance tubes by weight to within the tolerance specified by the manufacturer. Opposing tubes must be of equal weight. Use a laboratory balance for precise balancing.
- Loading: Load the rotor symmetrically. For partial loads, distribute tubes evenly. Secure the rotor lid if applicable.
- Run: Close the lid securely. Set the speed, time, and temperature. Start the centrifuge and wait until it reaches full speed before leaving the area. Never attempt to open the lid while the rotor is spinning.
II. Safety
- Never operate the centrifuge without the lid securely closed and the interlock engaged
- Do not exceed the maximum speed rating of the rotor or tubes
- If unusual noise or vibration occurs, stop the run immediately
- Inspect tubes for cracks or damage before each use
III. Maintenance
- After Each Use: Clean the rotor and chamber. Inspect the rotor for corrosion, cracks, or damage.
- Weekly: Clean and lubricate rotor O-rings and seals. Inspect the lid interlock for proper function.
- Monthly: Verify speed calibration using a tachometer. Check and replace brushes in brush-type motors.
- Annually: Professional service including bearing inspection, motor check, and full calibration.
