The Countleader FL 2000 is a fully automated, high-throughput cell counter designed to meet the  demands of multi-sample analysis. Using the patented FL24-Plate with microfluidic channels, the system  supports automatic staining. It enables dual-fluorescence staining (AO/PI) analysis for 24 samples in just 17  minutes (12 minutes for trypan blue staining).

Automatic Staining Technology

FL24-Plate-A features a unique design with pre-embedded  dyes. The AO/PI dyes are preloaded in the microfluidic chan nels. Simply add 40 µL of cell sample per well, and as the  cells flow through the microfluidic channels, staining and  analysis are performed automatically. This is ideal for  high-precision counting of a large number of samples that  need to be measured daily."

FL24-Plate-B is an empty 24-channel consumable plate.  Add 20 µL of cell sample and 20 µL of trypan blue dye  per well. The sample is automatically mixed and stained  as it flows through the microfluidic channels. This allows  for the analysis of 24 samples in just 12 minutes, making  it perfect for cell counting during process optimization  phases with a high number of daily samples

• The patented design of the microfluidic channels eliminates errors  caused by manual staining, ensuring precise and reliable counting  results  while making your work more efficient! The disposable FL24-Plate keeps  all samples confined within the consumable, with no flow paths in the  machine. Therefore no cleaning or maintenance is required after use.

• Each FL24-Plate is equipped with a unique QR code to  accurately record and calibrate the analysis volume of each well,  ensuring even greater  precision in results.

Simple Operation Workflow

Sample Loading

AO/PI Mode:only 40 µL of cell sample per well is required. Compatible with multichannel  pipettes, no pre-staining needed.

Trypan Blue Mode: only 20 µL of cell sample and 20 µL of trypan blue dye per well, with no  manual staining required.

Loading the Plate

Place the FL24-Plate into the designated slot of the Countleader FL 2000, select the wells to  be counted, and start the analysis.

Automatic Staining and Analysis

Samples are stained automatically as they flow  through the microfluidic channel. The fully auto mated system quickly delivers cell concentration, viabili ty, and both bright-field and fluorescence images  for all 24 samples with precision.

• Viable Cell Concentration

• Dead Cell Concentration

• Total Cell Concentration

• Cell Diameter

• Cell Aggregation Ratio

• Cell Fluorescence Images

• Cell Fluorescence Intensity Distribution Charts

• Cell Diameter Distribution Charts

Powerful and Flexible Software

The software features a flexible and user-friendly system interface with customizable detection channels.

• Typan blue and AO/PI modes are available

• Larger analysis volume reduces statistical errors

• The data can be searched by experiment name, time, and sample ID

• Separate sample ID facilitate results browsing and searching

• Dilution Factor is compensated automatically

• Growth curves aid in understanding and optimizing cell culture conditions

Accurate Data

The Countleader FL2000 ensures precise results through its automatic staining and analysis process, eliminating  errors caused by manual staining. With 1.5X magnification, the analysis area per field of view measures approxi mately 4.7 × 3.1 mm²—nearly 12 times larger than the traditional Hemocytometer method. Tens of thousands of  cells can be detected in a single sample, significantly reducing counting errors and ensuring reliable results.

CHO cell concentration gradient plot

•  Quickly obtain clear and accurate information on total cell  concentration, viable cell concentration, dead cell concentration,  cell concentration, cell diameter, and cell aggregation ratio.

• Use the Analysis Manager to view detailed data for each sample.

• Data can be exported in PDF, Excel, TIFF format.

Polygons and markers can be drawn directly on plots for precise gating and counting of subpopulations. The  software also provides the percentage of cells with varying diameters, offering insights into cell status, such as  stem cell differentiation and viral infection efficiency.

The software offers robust functionality to ensure accurate cell counting. Cells can be gated to verify proper  recognition and counting, guaranteeing precise results.

AO/PI fluorescence image A: original image; B: Magnified image, Gated cells are checked and recognized correctly

Continuously cultured cell samples can be moni tored and visualized as cell growth curves,  including:

Concentration curve

Viability curve

It is easy to track the cell growth state and trends  of cells, which is conducive to the optimization of  cell culture technology.

Compliant with 21 CFR Part 11

The Countleader Series offers three levels of login permissions, meeting GMP  requirements under 21 CFR Part 11. Key features include:

• Secure user login

• Multi-level user permissions

• Audit trails

• Log files

• Electronic signatures

IQ/OQ/PQ Verification

IQ, OQ, and PQ (3Q) certified services are available, with a variety of concentration beads and accompanying  verification documents for purchase. The 3Q operating manuals ensure consistent procedures, generate standard ized PDF reports, fully document sample usage, and meet audit trail requirements.

Compatible with various consumables

Applications

In CLD and PD departments, where a high volume of cell  samples requires counting, the workload can be overwhelm ing. The Countleader FL2000 provides a high-throughput  solution with low sample consumption, fast processing, and  automated staining and analysis.

• CLD department in Antibody and CGT field

• PBMC, Car-T in cell therapy

• Stem Cells

• Cancer Cells

• and more